Abstract
Quantitative phase imaging (QPI) has a very important application in the nondestructive imaging of biological cells. In view of the complex structure and various devices of imaging optical structure in traditional dual-channel orthogonal QPI, a new structure of a special pentaprism is designed as the main body of the orthogonal dual-channel phase microscopic imaging based on simulation design. In this dual-channel orthogonal QPI light structure, a hollow channel is designed as a sampling channel inside the pentaprism, a single light source through deflection of the pentaprism can form orthogonal dual-channel beams, with optical lens after diffraction modulation can form orthogonal dual-channel interference, the orthogonal dual-channel wrapped phase microscopic images are imaged on the sensors. The emulational and experimental results show that this design greatly simplifies the optical structure, uses very few optical devices, and the imaging path structure is compact and stable. With the use of single light source, the imaging quality of the dual light path has been significantly improved, which can provide a new technical basis for the improvement of biological cell (3-D) imaging technology and its instrument advancement.
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