New spreader for the preparation of gradient layers in thin-layer chromatography

Abstract

The preparation of gradient layers with reproducible separating properties suffers from various difficulties, so the Desaga gradient spreader, which has been known since 1964, has been redesigned. The new spreader allows the suspension to be passed directly through a special dividing wall into the cylindrical trough where mixing takes place. The layers thus prepared are highly sensitive and yield reproducible results. If chromatography is carried out transverse to the gradient (T-gradient), sensitive and substance-specific curves are obtained; chromatography parallel to the gradient improves separations to give selective focusing.