Abstract
The use of allergenic agents in wines needs to be monitored by both producers and supervisory authorities for the protection of sensitive individuals. Currently established as the gold standard is the ELISA-technique which relies on the affinity and specificity of antibodies. Since antibodies are produced in animals and current legislative developments demand the reduction of the use of animals for scientific purposes, the biosensor technology could be interesting for the quantification of allergens. By the combination of a technical device, the surface plasmon resonance, and a specific antibody it was possible to develop an antibody-based analytical method which is capable to detect lysozyme in quantities below 0.25 ppm. Since the biosensor is reusable multiple times, this approach can contribute to reduce the antibody consumption and therefore the use of animals for analysis. Furthermore, first insights in the use of new molecular receptors, aptamers were gained.
Highlights
The use of allergenic agents in wines needs to be monitored by both producers and supervisory authorities for the protection of sensitive individuals
An allergy is a reaction of the immune system to normally harmless substances, e.g. food components
Specific antibodies are produced by the immune system so that every following contact triggers the adverse reactions
Summary
An allergy is a reaction of the immune system to normally harmless substances, e.g. food components. Specific antibodies are produced by the immune system so that every following contact triggers the adverse reactions. To protect sensitive individuals, the EU legislation demands the labelling of food (including wine) which contains allergens listed in EU regulation 1169/2011 annex II, both as a trace or as an ingredient [3]. A labelling (use of terms or pictograms according to commission implementing regulation 579/2012) of ovalbumin and lysozyme in wine is necessary since exceptions are no longer applicable [4]. According to EU regulation 1308/2013 in combination with OIV/OENO 427/2010 and OIV-COMEX 502-2012, an amount of allergens above 0.25 ppm requires a labelling [5,6,7]
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