Abstract
In this present paper, we investigate the development of simple, rapid, accurate, reproducible and sensitive methods for the determination of serotonin (5-HT) in urine. For this purpose stationary fluorescence was used as method. With regard to the analysis of serotonin, the liquid-liquid extraction (LLE) solid phase extraction (SPE) and standard addition procedures were used. Several physicochemical factors affecting the sensitivity of the fluorescence intensity of serotonin were optimized, including the system of solvent (organic, micellar), the pH and the salts. The study of the analytical performances of the method led to very low limits of detection (LOD) varying between 0.1 and 3 ng/mL and to limits of quantification (LOQ) ranging between 0.4 and 10 ng/mL. This confirms the sensitivity of the method. Thus the low values of standard deviations (DRS) (between 0.3 and 6.6%) testify the good reproducibility of the measurements with satisfactory covering rate (89 to 111%). Accordingly, our results show that the spectrofluorimetric method is simple, fast and sensitive and can be applied to the routine analysis and does not require expensive equipment nor tiresome chemical pretreatments.
Highlights
Serotonin (5-HT) is a monoamine neurotransmitter that is primarily found in the gastrointestinal tract and central nervous system
We have developed in this work a simple, sensitive, accurate and inexpensive method for the determination of serotonin in urine samples
We have demonstrated the utility of the spectrofluorimetric analysis with the optimization of the fluorescence of 5-HT in the binary mixture water/methanol (1:1, v/v)
Summary
Serotonin (5-HT) is a monoamine neurotransmitter that is primarily found in the gastrointestinal tract and central nervous system. Serotonin was originally discovered by Vittorio Erspamer in 1935 (Qin, 2013). It was isolated, chemically identified as 5-hydroxytryptamine (5-HT), and named by Rapport and al. Identified as a vasoconstrictor substance in blood serum, approximately 80-90 percent of the human body’s total serotonin is located in the enterochromaffin cells in the gut, where it is used to regulate intestinal movements. The remainder is synthesized in serotonergic neurons in the Central Nervous System and in blood platelets.
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