New species of Chalara occupying coniferous needles

Abstract

Chalara is a genus of anamorphic fungi with typical phialidic conidiogenous cells. Species of Chalara live mostly as litter saprotrophs, many of them on coniferous litter. In this study, the morphology and DNA sequences (ITS rDNA, 28S rDNA and EF-1α) of strains of various species of Chalara isolated from coniferous litter needles were compared with those of strains from public collections. The majority of the isolates belonged to the C. longipes. Other strains represent C. piceae-abietis, C. microspora, C. microchona and four hitherto undescribed species. These are introduced here as C. holubovae sp. nov., C. hyalocuspica sp. nov., C. pseudoaffinis sp. nov. and C. recta sp. nov. Chalara recta is most closely related to C. longipes, which was found to be paraphyletic. However, no correlation of molecular data with the morphology was found. Chalara holubovae is specific among the Chalara species in that it has a synanamorph with fusiform conidia. Together with C. hyalocuspica, C. holubovae likely belong to the anamorphic Hyaloscyphaceae. Chalara piceae-abietis is epitypified. Epitypicifation of C. longipes and C. microspora cannot be done due to absence of a convenient specimen. Chalara austriaca may be re-discovered also after a targeted sampling in the locality of the type specimen. The majority of studied species are saprotrophic and colonise litter needles. An endophytic phase in living needles or other parts of a tree was confirmed for the species C. longipes and C. hyalocuspica. C. holubovae has been recorded only as an endophyte.