New Span-PEG-composited Fe3O4-CNT as a multifunctional ultrasound contrast agent for inflammation and thrombotic niduses.

Abstract

By attaching ferroferric oxide (Fe3O4) to drug-carrying carbon nanotubes (CNTs), we generated a new Span-PEG composite with Fe3O4-CNT multifunctional microbubbles for inflammation and thrombus niduses. The Fe3O4-CNT magnetic targeting complex was prepared by in situ synthesis, and then acetylsalicylic acid (ASA) and gentamicin (GM) were loaded onto the Fe3O4-CNT complex by physical methods to produce Fe3O4-CNT-ASA and Fe3O4-CNT-GM complexes, respectively. Span-PEG-composited Fe3O4-CNT-ASA or Fe3O4-CNT-GM microbubbles were synthesized with Span and PEG as the membrane materials by the acoustic cavitation method. The obtained composite microbubbles were smooth, hollow spheres with an average particle size of 425 nm. The ASA and GM loading rates in Span-PEG-composited Fe3O4-CNT-ASA and Fe3O4-CNT-GM microbubbles were 1.12% and 19.05%, respectively. Span-PEG-composited Fe3O4-CNT-ASA microbubbles inhibited thrombosis and demonstrated an anticoagulation effect in vitro. Additionally, Span-PEG-composited Fe3O4-CNT-ASA microbubbles showed significantly enhanced ultrasound imaging of rabbit abdominal aorta and extended the signal time under the action of an external magnetic field. Thus, Span-PEG-composited Fe3O4-CNT-GM microbubbles inhibited Escherichia coli and Staphylococcus aureus, enhanced the ultrasound imaging of rabbit abdominal uterus and had better stability and fluidity.