Abstract
Arbutin (4-Hydroxyphenyl-β-D-glucopyranoside) is a mono glycosidic form of hydroquinone and has been widely used in cosmetics for a long time due to skin whitening and freckle removing properties. In this study, arbutin was isolated from the methanol extract of Onobrychis galegifolia using flash chromatography. The structure of arbutin was elucidated by 1H and 13C -NMR spectroscopy and as well as 2D NMR techniques. In addition, quantitative analysis of arbutin found in O. galegifolia and O. nitida species was done using HPLC-DAD. According to the results, the arbutin content of methanol extracts of O. galegifolia and O. nitida was found 212.02 and 196.46 mg/g extract respectively. In addition, the arbutin content of cold-water extracts of O. galegifolia and O. nitida was found 128.2 and 99.40 mg/g extract. The amount of arbutin in dry weights was calculated for about 5.66% to 6.86%.
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