Abstract
A simplified procedure is described which permits employment of the Bush method for the separation of C-21 steroids on paper chromatograms at room temperature. Several new solvent systems have been devised, in which tert-butanol or a combination of tert-butanol and methanol constitutes the stationary phase. The new systems, used in combination with modified Bush systems, permit increased resolution of various steroid mixtures and increased structural characterization of unknown steroids, on the basis of their changing chromatographic behavior.
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