Abstract
A simple, low-cost technique has been developed for the rapid fabrication of single-layered paper-based microfluidic devices (μPADs). This technique, for the first time, made use of the deposition of patterned adhesive tape into the filter paper to construct hydrophobic barriers, with the help of toluene. Unlike other reported multi-layered μPADs that merely made use of adhesive tape as a separate layer for sealing or fluid flow controlling, the patterned adhesive tape was simultaneously dissolved and penetrated into the filter paper, which resulted in the successful transfer of the pattern from the tape to the filter paper. To demonstrate the effectiveness of this approach, nitrite and glucose were individually measured; detection limits as low as 0.015 ± 0.004 mM and 0.022 ± 0.006 mM were reported for nitrite and glucose, respectively. Multiplexed analysis of both analytes was also carried out with respective detection limits of 0.048 ± 0.005 mM and 0.025 ± 0.006 mM for nitrite and glucose. The application of the method was demonstrated by measuring nitrite and glucose in spiked artificial urine samples and satisfied recovery results were obtained.
Highlights
The images of the μPADs were obtained by a smart phone and analyzed as mentioned in solution containing both nitrite and glucose was added to the sample introduction zone, which the previousproduces subsection
We developed a new method for fabricating single-layered μPADs
The cut adhesive tape was dissolved by toluene and penetrated into the filter paper to form hydrophobic barriers, defining accurate areas of hydrophobicity and hydrophilicity
Summary
Paper-based microfluidic analytical devices (μPADs) have been widely used in the scientific community, since Whitesides group first proposed it in 2007 [1]. μPADs have a number of merits, such as ease of use, low volume, biodegradability, portability, disposability, and good biocompatibility [2]. Sensors 2019, 19, 4082 by the need for expensive instruments and trained personnel To solve such problems, some groups have developed simple techniques to construct μPADs. Cardoso et al developed microfluidic devices by stamping paraffin barriers in paper platform for the colorimetric determination of nitrite [24]. No work has been reported on utilizing the deposition of cut adhesive tape into the filter paper to fabricate single-layered paper microfluidics, which is a mask-free fabrication process without the usage of any expensive instruments (laser cutter, cutting plotter, screen printer, wax printer, 3D printer, etc.). Nitrite and glucose were used as the analytes to demonstrate the assay performance of the developed μPADs. In this paper, a simple, low-cost and highly-adaptable prototyping process for the fabrication of single-layered μPADs has been developed. Recovery experiments were carried out by analyzing the concentrations of nitrite and glucose in artificial urine samples
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