New Rhizobium leguminosarum bv. trifolii isolates: Evaluation of competitiveness for clover nodule occupancy

Abstract

An interrelationship between introduced and indigenous rhizobia focused on their competitiveness in nodulation was evaluated in a soil pot experiment. Clover seeds were inoculated by six different gradual concentrations of inoculum of two effective Rhizobium leguminosarum bv. trifolii isolates (inoculation strains 1/2 and 14/2). At the beginning of flowering, clover plants were removed from the pots, and nodules from each pot representing different degrees of inoculum level were taken for reisolate cultivation. The PCR technique was used for the identification of rhizobial reisolates, random amplified polymorphic DNA product patterns were acquired and analysed. Nodule occupation by inoculation strains or indigenous Rhizobium leguminosarum bv. trifolii strains was assessed by comparing the number of nodules formed by inoculation or indigenous strains and inoculation strains competitiveness was calculated. Nodule occupancy by the inoculation strain 1/2 increased sharply with the increase in inoculum dose, whereas in inoculation strain 14/2 more nodules were formed gradually starting from low inoculum level. Competitiveness of inoculation strain 1/2 was calculated as low and was documented by an absence of nodule occupancy in four inoculation levels. On the other hand, competitiveness of the inoculation strain 14/2 was considerably higher, and even in low inoculum dose this strain was more competitive than native rhizobia. Although both the inoculation strains 1/2 and 14/2 were found highly efficient in nitrogen fixation, only the strain 14/2 was able to manifest this characteristic due to the higher competitiveness when applied in lower doses.