Abstract
We document the first known occurrences of the Crystal Darter (Crystallaria asprella) in the middle Mississippi River (MMR). Two young-of-the-year specimens were collected, the first on 6 June 1998 at river mile (RM) 78 and the other on 9 June 2004 at RM 55. Both specimens were collected in side channels at depths of 1 m and 5.4 m, respectively.
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