Abstract
ABSTRACT We analyzed the presence of Leptospira spp. in liver and kidney tissue of wild marsupials and rodents trapped in a periurban forest in the eastern Brazilian Amazon. We examined 25 individuals of four marsupial and seven rodent species for the presence of the 16S rRNA gene of Leptospira in the DNA extracted from 47 liver and kidney tissue samples using PCR. We detected positive samples in 12% (3/25) of the individuals, in kidney fragments of two marsupial species (Didelphis marsupialis and Marmosops pinheiroi) and in a liver fragment of one rodent species (Echimys chrysurus). These are the first records of Leptospira spp. in M. pinheiroi and E. chrysurus and it is the first molecular survey of marsupials and rodents in the Brazilian Amazon.
Highlights
We analyzed the presence of Leptospira spp. in liver and kidney tissue of wild marsupials and rodents trapped in a periurban forest in the eastern Brazilian Amazon
Novos registros de Leptospira spp. em marsupiais e um roedor silvestres na Amazônia Oriental por detecção por PCR
Estes são os primeiros registros de Leptospira spp. em M. pinheiroi e E. chrysurus, e é o primeiro levantamento molecular de marsupiais e roedores na Amazônia brasileira
Summary
We analyzed the presence of Leptospira spp. in liver and kidney tissue of wild marsupials and rodents trapped in a periurban forest in the eastern Brazilian Amazon. New records of Leptospira spp. in wild marsupials and a rodent in the eastern Brazilian Amazon through PCR detection.
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