New records of fungal pathogens of invertebrates from endemic pine forests in Mexico

Rebeca Casique-Valdés,Sten Anslan,Sergio R Sanchez-Peña,Fernando Galindo-García

doi:10.15560/18.1.67

Abstract

We report fungal pathogens of invertebrates (FPI) (Entomophthorales, Hypocreales, and Orbiliales) from roots of an endemic Mexican pine, Pinus greggii Engelm. ex Parl., at four primary montane forests using next-generation sequencing. We found twenty-nine OTUs from 18 genera of FPI associated to the roots of P. greggii. New records for Mexico are: Beauveria felina (DC.) J.W.Carmich., Dactylella mammillata S.M. Dixon, Dactylella ramosa Matsushima, Drechslerella brochopaga (Drechsler) M. Scholler, Hagedorn &amp; A. Rubner, Hirsutella minnesotensis Chen, Liu &amp; Chen, Leptobacillium leptobactrum (W.Gams) Zare &amp; W.Gams, Metapochonia variabilis Z.F.Zhang, F.Liu &amp; L.Cai, Monacrosporium leptosporum (Drechsler) A. Rubner, and Simplicillium aogashimaense Nonaka, Kaifuchi &amp; Masuma. A largely unknown array of fungal pathogens of invertebrates are likely to be found in Mexican forests. &nbsp;This work facilitates future analyses of fungal diversity in these primary forests, as well as basic and applied research in biological control.

Highlights

Soil, including the rhizoplane and roots, is the natural habitat and reservoir for many fungal pathogens of invertebrates (FPI)
We report fungal pathogens of invertebrates (FPI) (Entomophthorales, Hypocreales, and Orbiliales) from roots of an endemic Mexican pine, Pinus greggii Engelm. ex Parl., at four primary montane forests using next-generation sequencing
We found a total of 2081 sequences in 29 OTUs from 18 genera of FPI associated to the roots of Pinus greggii

Summary

Introduction

Soil, including the rhizoplane and roots, is the natural habitat and reservoir for many fungal pathogens of invertebrates (FPI). These fungi play a crucial role in the population dynamics of their hosts and are important in biological control and sustainable management of invertebrate pests (Toepfer et al 2010). Their hosts include many ecdysozoans: arthropods, nematodes, and tardigrades. Forests are reservoirs of FPI (Sánchez-Peña et al.2011). There is little information on FPI from natural pine forests in Mexico. We profiled the community structure of FPI in a Mexican pine, Pinus greggii Engelm. ex Parl., forest and indicate the systematic and trophic position of

Methods

Results

Conclusion