New records and review of the occurrence of the oriental hornet Vespa orientalis Linnaeus, 1771 in the islands of Greece (Hymenoptera: Vespidae: Vespinae)

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Records of Vespa orientalis Linnaeus, 1771 for 28 islands of Greece are given: in 18 islands (Agistri, Euboea, Folegandros, Kalamos, Kalymnos, Karpathos, Kos, Leros, Lefkada, Naxos, Nisyros, Paros, Patmos, Pserimos, Telendos, Tilos, Tinos, Sifnos), these occurrences are the first record of the species for them. The total number of Greek islands in which V. orientalis is known becomes 33.

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  • Preprint Article
  • 10.21203/rs.3.rs-6179679/v1
Rethinking spillover risks: first description of the Vespa orientalis gut microbiome and its impact on honeybee and human health
  • Apr 22, 2025
  • Simone Cutajar + 7 more

Abstract Vespa orientalis (Oriental hornet) is expanding its range worldwide, raising concerns about its predatory impact on honey bees and potential health risks to humans. This study provides the first comprehensive description of the V. orientalis gut microbiome, explores how diet and location influence microbial composition, and bee pathogens reservoir. Adult hornets with different feeding behaviors were sampled from four urban and natural sites in Malta. 16S rRNA gene sequencing revealed a gut microbiota dominated by Firmicutes and Proteobacteria, with key genera including Carnimonas, Arsenophonus, and Rosenbergiella. Significant compositional shifts were observed in relation to diet and sampling location, suggesting that environment and diet significantly shape the hornet gut community. Moreover, detection of certain honey bee-associated microbes, such as Gilliamella and Snodgrassella, points to potential microbial exchange between predator and prey. Quantitative PCR targeting bee and human pathogens detected high prevalence rates of Nosema ceranae, Crithidia bombi, and Serratia, while Listeria, Salmonella, and other bee pathogens were absent. Our findings suggest V. orientalis may function more as a pathogen reservoir rather than a primary spillover vector and lays the groundwork for targeted management strategies to mitigate its impact on apiculture and broader ecosystem services.

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  • Research Article
  • Cite Count Icon 8
  • 10.1002/vms3.1310
The oriental hornet (Vespa orientalis) as a potential vector of honey bee's pathogens and a threat for public health in North-East Italy.
  • Nov 1, 2023
  • Veterinary Medicine and Science
  • Paolo Zucca + 15 more

Oriental hornets are large predatory hymenoptera that occur in the southern part of Asia and the southeastern Mediterranean. Among many pests of bee colonies, Vespa orientalis was recorded to be one of the most destructive. The aim of this study was to: (1) monitor the presence of pathogens carried by V. orientalis that could potentially threaten honey bees and public health; (2) describe the hornet's predatory behavior on honey bee colonies and (3) collect the medical history of a V. orientalis sting suffered by a 36-year-old woman. Observations of V. orientalis predatory behavior and the catches of hornets for parasitological and microbiological examination, using molecular and bacteriological analyses, were carried out in three experimental apiaries, both in spring in order to capture the foundress queens and during the summer to capture the workers. Furthermore, the medical history and photographic documentation of a V. orientalis sting suffered by a 36-year-old woman have been collected. The results obtained highlight that V. orientalis is capable of causing serious damage to beekeeping by killing bees, putting under stress the honey bee colonies and by potentially spreading honey bee pathogens among apiaries. These hornets may also become a public health concern, since they are capable of inflicting multiple, painful stings on humans. Only the development of an Integrated Management Control Program will be able to contain the negative effects of anomalous population growth and the potentially negative impact on honey bees and public health of V. orientalis.

  • Research Article
  • 10.1186/s42523-025-00460-6
Gut microbiome of Vespa orientalis: functional insights and potential honey bee pathogen dynamics
  • Sep 30, 2025
  • Animal Microbiome
  • Simone Cutajar + 7 more

Vespa orientalis, the oriental hornet, is an emerging predator of honey bees whose ecological impact and microbial ecology remain poorly understood. Here, we present the first detailed characterisation of its gut microbiota by integrating 16S rRNA gene sequencing, predicted microbial function, pathogen screening, and a three-year beekeeper survey across urban and rural sites in Malta. Hornets were sampled from four locations and classified by observed foraging behaviour, either predation on honey bees or scavenging on cat food.Survey data confirmed consistent V. orientalis sightings and seasonal colony losses, particularly during peak foraging months. Microbiome analysis revealed a conserved core community dominated by Spiroplasma, Arsenophonus, and Rosenbergiella, with overall diversity stable across sites and diets. However, specific taxa varied with foraging behaviour. For example, Arsenophonus was enriched in bee-predating hornets, while Enterobacter and Serratia were more common in scavenging individuals, suggesting environmental and dietary influences on microbiota composition. Predicted functional profiles remained broadly conserved, reflecting robust nutrient metabolism and potential detoxification capabilities, with some variations related to the diet behaviour.Pathogen screening detected Nosema ceranae and Crithidia bombi in a substantial proportion of hornets, including those not observed feeding on bees. Although our findings do not demonstrate pathogen transmission, they support the hypothesis that V. orientalis may act as a transient carrier, potentially contributing to pathogen persistence via environmental exposure.Together, these results reveal the dietary flexibility and microbial flexibility within the gut microbiome of V. orientalis, and highlight its potential involvement in pollinator pathogen dynamics.Supplementary InformationThe online version contains supplementary material available at 10.1186/s42523-025-00460-6.

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Distribution pattern of the hornets Vespa orientalis and V. crabro in Iran
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  • Ebrahim Ebrahimi + 1 more

Two species of hornets are present in Iran: Vespa orientalis Linnaeus, 1771, has a widespread distribution in most parts of Iran, except for the Caspian coast in northern Iran, but V. crabro Linnaeus, 1758, is present only on the Caspian coast. The ambiguity regarding these two species in Iran, their distribution patterns, diagnoses and agricultural aspects are discussed.

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  • Cite Count Icon 4
  • 10.14258/abs.v4i1.3915
On the expansion of the areas of Vespa orientalis and Polistes wattii (Hymenoptera: Vespidae) on the territory of the Republic of Kazakhstan
  • Mar 18, 2018
  • Acta Biologica Sibirica
  • I I Temreshev

The new data received on the distribution of two species of social wasps in the territory of the Republic of Kazakhstan-the eastern, or Vespa orientalis Linnaeus, 1771 (Oriental hornet), and the Polistes wattii Cameron, 1900. Currently in territory of Almaty oblast there is a stable self-reproducing population of the V. orientalis and its range is constantly expanding. On the territory of the Zhambyl oblast, it was previously recorded in the Talas district, but for Shu and Sarysu districts not indicated. In the Kyzylorda oblast, it was previously recorded in the Syrdarya district, for the Zhanakorgan district not specified. In general, it not specified earlier for the Karaganda oblast. Thus, in general, the territory of Kazakhstan is expanding its range. P. wattii in Kazakhstan was observed relatively recently (2012, the villages of Shardara and Asykata in Shardara and Maktaaral districts of the South Kazakhstan oblast). North of the indicated points of South Kazakhstan and in the Kyzylorda and Zhambyl oblasts, it was not previously indicated. Apparently, the species gradually expands its area within the Republic of Kazakhstan. Both species are of practical importance as pests of agriculture and beekeeping, and sanitary and epidemiological and medical - as poisonous animals. In addition, their introduction into new habitats can disrupt the existing ecological balance. Proceeding from, is necessary to monitor further spreading of these wasps within Kazakhstan, and to start working on the issue of measures to limit their numbers taking into account world experience.

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  • 10.9734/jalsi/2021/v24i230219
Quantitative and Qualitative Impacts of Selected Arthropod Venoms on the Larval Haemogram of the Greater Wax Moth, Galleria mellonella (Linnaeus) (Lepidoptera: Pyralidae)
  • Apr 11, 2021
  • Journal of Applied Life Sciences International
  • Dyaa Emam + 3 more

The greater wax moth, Galleria mellonella (Linnaeus) (Lepidoptera: Pyralidae) is the most destructive pest of honey bee, Apis mellifera Linnaeus (Hymenoptera: Apidae), throughout the world. The present study was conducted to determine the quantitative and qualitative impairing effects of the arthropod venoms, viz., death stalker scorpion Leiurus quinquestriatus (Hemprich & Ehrenberg) venom (SV), oriental Hornet (wasp) Vespa orientalis Linnaeus venom (WV) and Apitoxin of A. mellifera (AP) on the larval haemogram. For this purpose, the 3rd instar larvae were treated with LC50 of each of these venoms (3428.9, 2412.6, and 956.16 ppm, respectively). The haematological investigation was conducted in haemolymph of the 5th and 7th (last) instar larvae. The important results could be summarized as follows. Five basic types of the freely circulating haemocytes in the haemolymph of last instar (7th) larvae of G. mellonella had been identified: Prohemocytes (PRs), Plasmatocytes (PLs), Granulocytes (GRs), Spherulocytes (SPs) and Oenocytoids (OEs). All venoms unexceptionally prohibited the larvae to produce normal hemocyte population (count). No certain trend of disturbance in the differential hemocyte counts of circulating hemocytes in larvae of G. mellonella after treatment with the arthropod venoms. Increasing or decreasing population of the circulating hemocytes seemed to depend on the potency of the venom, hemocyte type and the larval instar. In PRs of last instar larvae, some cytopathological features had been observed after treatment with AP or WV, but SV failed to cause cytopathological features. With regard to PLs, some cytopathological features had been observed after treatment with AP while both SV and WV failed to cause cytopathological features in this hemocyte type. No venom exhibited cytopathological effects on GRs, SPs or OEs.

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  • 10.1111/j.1096-3642.1947.tb00131.x
The Edibility of Birds: Illustrated by Five Years' Experiments and Observations (1941–1946) on the Food Preferences of the Hornet, Cat and Man;and considered with Special Reference to the Theories of Adaptive Coloration
  • Feb 1, 1947
  • Proceedings of the Zoological Society of London
  • Hugh B Cott

The Edibility of Birds: Illustrated by Five Years' Experiments and Observations (1941–1946) on the Food Preferences of the Hornet, Cat and Man;and considered with Special Reference to the Theories of Adaptive Coloration

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Export Marketing Communications of Public Sector in Promoting Tourism Destination of a Greek Island
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  • Front Matter
  • Cite Count Icon 28
  • 10.1016/j.jpeds.2015.10.060
The “Invisible Children”: Uncertain Future of Unaccompanied Minor Migrants in Europe
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  • The Journal of Pediatrics
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The “Invisible Children”: Uncertain Future of Unaccompanied Minor Migrants in Europe

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Molecular phylogeny and identification of the Egyptian wasps (Hymenoptera: Vespidae) based on COI mitochondrial gene sequences
  • Apr 5, 2018
  • Egyptian Journal of Biological Pest Control
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  • Jul 1, 2023
  • IOP Conference Series: Earth and Environmental Science
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  • 10.36077/kjas/2023/v15i2.10391
The effect of some food sources and hive products on the oriental hornet Vespa orientalis Linnaeus 1771 (Hymenoptera: Vespidae)
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  • Kufa Journal for Agricultural Sciences
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The last checklist of the water mites of the Balkan countries published in 2010 by Pešić et al. is updated to November 2017. This supplement includes new records of water mite species from the Balkan countries (Croatia, Bosnia Herzegovina, Montenegro, Albania, Macedonia, Serbia, Bulgaria and Greece) published after 2010, as well as unpublished records based on material collected from Croatia, Montenegro, Albania, Macedonia, and Greece. Numerous new records for the national faunas, including one species new for the Mediterranean region (Arrenurus stjordalensis Thor, 1899), are reported and one species new to science (Trichothyas jadrankae Pešić sp. nov.) is described. With these additions, a total number of 390 water mite species and subspecies from 34 families and 77 genera is now recorded from the Balkan peninsula (including the Greek Islands).

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  • 10.3372/wi.36.36123
Floristic and phytosociological investigation of the island Antikythera and nearby islets (SW Aegean, Greece)
  • Feb 27, 2006
  • Willdenowia
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The Mediterranean refugee crisis: ethics, international law and migrant health
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Europe is experiencing levels of forced migration not seen since the Second World War. Its sources lie in the fragile, strife-torn states of the Middle East and Africa: four million...

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