New pyrazolo[4,3-e][1,2,4]triazine sulfonamides as carbonic anhydrase inhibitors

Abstract

New sulfonamide derivatives of pyrazolo[4,3-e][1,2,4]triazine were synthesized and investigated as antitumor agents through carbonic anhydrase (CA, EC 4.2.1.1) inhibition. The newly prepared compounds were tested for their anticancer activity against human breast cancer cell lines (MCF-7, MDA-MB-231) using a MTT assay and inhibition of [3H]thymidine incorporation into DNA. Preliminary biological studies with several CA isoforms, revealed that the new derivatives were ineffective as CA I and II inhibitors, but they showed activity against tumor-associated enzymes, such as CA IX. The most effective inhibitor against CA IX was compound 8e that exhibited an inhibition constant KI of 13.8nM, and derivatives 8c–8d with moderate activity (8c: KI=25.4nM; 8d: KI=24.5nM). Compounds 8g–8h exhibited acceptable activity (8g: KI=27.7nM; 8d: KI=26.6nM). The detailed synthesis, spectroscopic and biological data are also reported.