Abstract
Abstract A Gentle method of group separation of low molecular weight hydrophilic natural products is reported. The method is based on separation of the compounds according to their net charge at different pH values using different types of ion-exchange columns connected in series. Precolumns retaining interfering compounds are used in some cases. Elution of the compounds retained on the columns is performed by use of volatile eluents. The elution principle for two of the ion-exchangers in question is removal of the charges on the column materials while for the third column the positive net charge on the compounds retained is removed. Thereby, the total amount of ions retained on the different columns is released and eluted into small volumes, which after evaporation leaves the ions as well defined salts. The method is experimentally simple and efficient to separation of natural products into groups suitable to direct use in sensitive methods of analysis as e.g. high-performance liquid chromatography and g...
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