New Poly(Propylene Imine) Dendrimer Modified with Acridine and Its Cu(II) Complex: Synthesis, Characterization and Antimicrobial Activity

Paula Bosch,Rositsa Kukeva,Ivanka Nikolova,Ivo Grabchev,Desislava Staneva,Radostina Stoyanova,Petar Grozdanov,Evgenia Vasileva-Tonkova

doi:10.3390/ma12183020

Abstract

A second-generation poly(propylene imine) dendrimer modified with acridine and its Cu(II) complex have been synthesized for the first time. It has been found that two copper ions form complexes with the nitrogen atoms of the dendrimeric core by coordinate bonds. The new compounds have been characterized by nuclear magnetic resonance (NMR), electron paramagnetic resonance (EPR), fourier-transform infrared spectroscopy (FTIR) and fluorescence spectroscopy. The spectral characteristics of the modified dendrimer have been measured in different organic solvents, and a negative fluorescence solvatochromism has been observed. The antimicrobial activity of the dendrimers has been tested against model pathogenic microorganisms in agar and by broth dilution method. The cotton fabric treated with both dendrimers has been evaluated towards pathogenic microorganisms. The obtained modified cotton fabrics have been shown to hamper bacterial growth and to prevent biofilm formation. Dendrimer cytotoxicity has been investigated in vitro in the model HEp-2 cell line.

Highlights

Antibacterial surfaces are very important with regard to minimizing infectious diseases which are one of the main causes of mortality worldwide [1]
To obtain a polypropylene imine dendrimer modified with acridine fluorophores, we used a
To obtain a polypropylene imine dendrimer modified withgroups acridineinfluorophores, used a second-generation dendrimer containing eight primary amino its periphery,we enabling second-generation dendrimer containing eight primary amino groups in its periphery, enabling eight acridine fragments to be coupled into one molecule

Summary

Introduction

Antibacterial surfaces are very important with regard to minimizing infectious diseases which are one of the main causes of mortality worldwide [1]. This problem is mainly due to the increasing resistance of pathogenic microorganisms to antibiotics applied in clinical practice [2] Heterocyclic compounds have major role in the design and investigations of new bioactive drugs [3,4] acridine derivatives are one of the intensively exploited organic fluorophores in which fluorescence color intensity depends strongly on the polarization of their chromophoric system [5,6]. Compared to the low molecular weight bioactive compounds, dendrimers have the potential to deliver a large dose

Methods

Results

Conclusion