Abstract
New polymorphic SSR markers in sugarcane has potential usefulness to studies genetic diversity, genetic mapping, DNA fingerprinting and determination offspring as true hybrids, selfing or contaminant in germplasm from breeding program. SSR markers can be obtained in several ways, and the search for SSR-EST sequences an alternative with significant results. The aim of this study was to develop highly polymorphic SSR markers for germplasm studies in sugarcane. Di- tri- and tetra-nucleotide sequences were mined in the TGI bank and 53 EST-SSR markers developed. These markers were analyzed in five genotypes and showed to be highly polymorphic, with the PIC ranging between 0.48 and 0.95 with a mean of 0.86. The discriminatory power ranged from 0.35 to 0.94 with a mean of 0.82 and the genetic similarity was high in the accessions evaluated.
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