Abstract
Plant hormones play a crucial role in controlling plant growth and development. These groups of naturally occurring substances trigger physiological processes at very low concentrations, which require sensitive techniques for their quantitation.This study reports on the development of a newly synthesized polymer sorbent for the analysis of indole-3-acetic acid (IAA), which is the most important auxin in plants, from complex raw matrices, such as plant extracts. IAA was determined by high-performance liquid chromatography (HPLC) coupled with fluorescence detection (FD).The polymer developed was able to bind about 94% of IAA with a reproducible manner. The release of IAA in methanol was more than 70% for both of them. Non-specific hydrophobic interactions are, likely, the dominant driving force of the binding between IAA and the polymer.This methodology was applied for the determination of IAA in raw lemon leaves. The detection and quantification limits for IAA in real samples were 0.50±0.08ng/g and 2.64±0.09ng/g (n=3, α=0.05), respectively. As expected, the IAA concentration in uninfected lemon sample was significantly lower respect to the concentration found in infected sample.
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Disclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.