Abstract
Three macromutants namely, 'pigmented stem', 'viridis' and 'lax branching', of jute (Corchorus olitorius L. - JRO 524; commercial fibre yielding crop) were induced at M2 following gamma irradiations and EMS (Ethyl Methane Sulphonate) treatments. The true breeding mutants (selfed seeds of a single phenotypically stable plant forwarded in each generation from M4) are assessed considering different attributes like germination, survivability, meiotic chromosome behavior, pollen fertility and viability and quantitative traits across the generations (M5, M6 and M7) in relation to control under a uniform field condition(s). Further, genetic variations between/among the mutants and between mutant(s) and control is also analyzed from seed protein (SDS-PAGE - sodium dodecyl sulphate-polyacrylamide gel electrophoresis), RAPD (Random Amplification of Polymorphic DNA) and ISSR (inter-simple sequence repeat) profiles. Pigment profiles following TLC (Thin Layer Chromatography) have been studied in 'viridis' and control. Inheritance of the mutant trait(s) has also been assessed from reciprocal crossings performed between control and mutants. Results suggest that the mutants are true breeding, stable and significant and may enrich genetic resources in the species.
Highlights
Induced mutagenesis is a potent tool for widening gene pool in a species through creation of genetic variations in a short span of time
A good number of elite ‘plant type’ mutants are reported in C. capsularis[3,4,5,6] as well as in C. olitorius[4,5,7] but it is always desirable to raise superior mutants of economic worth by exploring the existing germplasm(s)
The objective of the work is to recommend new, stable and true breeding germplasm resources in C. olitorius that may be beneficial for commerce as well as for efficient breeding
Summary
Induced mutagenesis is a potent tool for widening gene pool in a species through creation of genetic variations in a short span of time. Induced mutation has contributed significantly to global agriculture by producing more than 3000 mutants with desirable qualitative and quantitative traits in about 175 crop species[1]. A good number of elite ‘plant type’ mutants are reported in C. capsularis[3,4,5,6] as well as in C. olitorius[4,5,7] but it is always desirable to raise superior mutants of economic worth by exploring the existing germplasm(s). Maity and Datta[8] raised 3 plant type mutants in C. olitorius namely, ‘pigmented stem’, ‘viridis’ and ‘lax branching’ following gamma irradiation and EMS treatments.
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