New Perspectives for Real-Time Multi-Metabolites Monitoring of Differentiating Neuron Precursors by using Protein-Based Carbon Nanotubes

Abstract

Event Abstract Back to Event New Perspectives for Real-Time Multi-Metabolites Monitoring of Differentiating Neuron Precursors by using Protein-Based Carbon Nanotubes Sandro Carrara1* 1 Ecole Polytechnique Federale de Lausanne, School of Engineering, Switzerland Monitoring of metabolic compounds, such as glucose and lactate, is extensively reported in literature, especially for clinical purposes. Instead, the application of such technologies for monitoring metabolites in cell cultures has not been deeply explored. Monitoring systems can provide further information to the current state-of-the-art of cell lines, particularly those that are not fully known, as stem and embryonic cells. On the other hand, metabolism biosensors can address fully automation for growing cell cultures, once sensors are coupled with fluidic systems for cell feeding. Among different presented strategies to develop biosensors, carbon nanotubes exhibit great properties, particularly suitable for bio-sensing. In this talk, nano-structured electrodes by using multi-walled carbon nanotubes are presented for specific detection of glucose, glutamate and lactate in neuron cultures. Some results from simulations are illustrated in order to show the behavior of carbon nanotubes depending on their orientation once they are casted onto electrodes surface. Data from Scanning Electron Microscopy, electrochemical amperometry and voltammetry are presented to characterize the performance of the developed sensors. Carbon nanotubes functionalized with proper probe proteins are characterized in terms of sensitivity, detection limit, and time stability. Their detection performances are compared with previously published results. The monitoring of cell cultures of neurons precursors is showed and metabolic behavior is registered as validation of the developed monitoring system. Glucose and lactate are acquired during proliferation of neuron precursors and their differentiation in mature neurons. We also investigate lactate and glucose in conditions of glucose deprivation, showing direct confirmation of lactate self-production during cell suffering. Finally, new perspectives in continuous real-time monitoring of cell metabolism are presented. The talk demonstrates that the proposed electrochemical biosensors based on carbon nanotubes are suitable for stem cell real-time monitoring as well as for cell biology investigation. Keywords: neuroengineering Conference: The Monte Verita' Workshop on the Frontiers in Neuroengineering, Ascona, Switzerland, 5 Sep - 9 Sep, 2010. Presentation Type: Oral Presentation Topic: Frontiers in Neuroengineering Citation: Carrara S (2010). New Perspectives for Real-Time Multi-Metabolites Monitoring of Differentiating Neuron Precursors by using Protein-Based Carbon Nanotubes. Front. Neuroeng. Conference Abstract: The Monte Verita' Workshop on the Frontiers in Neuroengineering. doi: 10.3389/conf.fneng.2010.10.00009 Copyright: The abstracts in this collection have not been subject to any Frontiers peer review or checks, and are not endorsed by Frontiers. They are made available through the Frontiers publishing platform as a service to conference organizers and presenters. The copyright in the individual abstracts is owned by the author of each abstract or his/her employer unless otherwise stated. Each abstract, as well as the collection of abstracts, are published under a Creative Commons CC-BY 4.0 (attribution) licence (https://creativecommons.org/licenses/by/4.0/) and may thus be reproduced, translated, adapted and be the subject of derivative works provided the authors and Frontiers are attributed. For Frontiers’ terms and conditions please see https://www.frontiersin.org/legal/terms-and-conditions. Received: 24 Aug 2010; Published Online: 10 Sep 2010. * Correspondence: Dr. Sandro Carrara, Ecole Polytechnique Federale de Lausanne, School of Engineering, Lausanne, Switzerland, sandro.carrara@epfl.ch Login Required This action requires you to be registered with Frontiers and logged in. To register or login click here. Abstract Info Abstract The Authors in Frontiers Sandro Carrara Google Sandro Carrara Google Scholar Sandro Carrara PubMed Sandro Carrara Related Article in Frontiers Google Scholar PubMed Abstract Close Back to top Javascript is disabled. Please enable Javascript in your browser settings in order to see all the content on this page.