New paradigm shifts in micropropagation of fruit crops through bioreactors - a review

Abstract

within relatively shorter periods, as well as significant reductions in hyperhydricity in plants as a result of efficient gas exchange, oxygen supplementation and automation, bioreactors, specifically temporary immersion systems (TIS), are being utilized for mass multiplication of forestry and horticultural crops. In tissue culture of banana, date palm, strawberrys, papaya, citrus, grape, pineapple, apple, pear, plum, chestnut, pistachio nut, apricot, sweet cherry, and almond, a variety of TIS bioreactors were used, including RITA, Plantform, SETIS and twin glass airlifts. TIS Bioreactors need to be improved in terms of space utilization. The space utilization was found to be highest with the Plantform system (80%) and lowest in the Twin Flask system (26%). Higher head space provides better plant growth and lesser fogging on the walls of the bioreactor. Most bioreactors have not been designed to facilitate better root production in vitro. Roots get coiled and cluttered, which needs improvement in design. The provision of illumination in each tank will facilitate better morphogenesis. This paper describes the micropropagation of fruit crops using different TIS bioreactors.