Abstract
DNA can adopt different conformations, such as B-DNA, Z-DNA and G4-quadruplex DNA. Each of these has a unique structure and different roles in cells. The 3D organization of the nucleus is complex, and plays a role in gene expression. We demonstrated, for the first time, the spatial genomic organization of three different DNAs concurrently in HeLa cells, and human paraffin-embedded tissue sections and frozen sections, using anti-B-DNA, anti-Z-DNA and anti-G4 DNA antibodies. Data was obtained by confocal microscopy using Alexa Fluor conjugated secondary antibodies [Alexa Fluor 405 (blue), Alexa Fluor 488 (green) and Alexa Fluor 555 (red)], along with wheat germ agglutinin conjugated with Alexa Fluor 647 (white) as a morphological counterstain.
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