Abstract
Thinopyrum has been widely used to improve wheat (Triticum aestivum L.) cultivars. Non-denaturing fluorescence in situ hybridization (ND-FISH) technology using oligonucleotides (oligo) as probes provides a convenient and efficient way to identify alien chromosomes in wheat backgrounds. However, suitable ND-FISH-positive oligo probes for distinguishing Thinopyrum chromosomes from wheat are lacking. Two oligo probes, Oligo-B11 and Oligo-pThp3.93, were designed according to the published Thinopyrum ponticum (Th. ponticum)-specific repetitive sequences. Both Oligo-B11 and Oligo-pThp3.93 can be used for ND-FISH analysis and can replace conventional GISH and FISH to discriminate some chromosomes of Th. elongatum, Th. intermedium, and Th. ponticum in wheat backgrounds. The two oligo probes provide a convenient way for the utilization of Thinopyrum germplasms in future wheat breeding programs.
Highlights
Thinopyrum intermedium (Th. intermedium), Thinopyrum ponticum (Th. ponticum) and Thinopyrum elongatum (Th. elongatum) are important reservoirs of elite genes for wheat (Triticum aestivum L.) breeding programs
Oligonucleotide probes combined with non-denaturing fluorescence in situ hybridization (ND-FISH) can be used to discriminate alien chromosomes in wheat backgrounds conveniently [10,11]
Using oligo probes combined with the ND-FISH assay, rye (S. cereale) and Dasypyrum villosum chromosomes can be effectively and accurately distinguished from common wheat chromosomes [4,10,11]
Summary
Thinopyrum intermedium (Th. intermedium), Thinopyrum ponticum (Th. ponticum) and Thinopyrum elongatum (Th. elongatum) are important reservoirs of elite genes for wheat (Triticum aestivum L.) breeding programs. Genomic in situ hybridization (GISH) and fluorescence in situ hybridization (FISH) technologies can be used to differentiate and localize Th. intermedium, Th. ponticum and Th. elongatum chromosomes in wheat backgrounds [1,2,3,4,5,6,7,8]. GISH and FISH are time-consuming because of the preparation and labeling of probe sequences, and denaturing of the probes and chromosomes [9,10]. Oligonucleotide (oligo) probes combined with non-denaturing fluorescence in situ hybridization (ND-FISH) can be used to discriminate alien chromosomes in wheat backgrounds conveniently [10,11]. Suitable ND-FISH-positive oligo probes for distinguishing Thinopyrum chromosomes from wheat are lacking.
Sign-in/Register to view highlights & summary Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
