Abstract
Corn, sorghum and wheat grains are used as livestock feed in the world. Identification of black aspergilli associated with these grains is necessary to make sure of the safety of the grains because its occurrence is an indicator of mycotoxin production. Forty-five isolateswere isolatedfrom the samples collected from Upper Egypt's markets and identified morphologically based on colony color,conidia,stipeand vesicle size and molecularly byusing β-tubulinandcalmodulingenes. Isolates were divided into 30 strains ofAspergilluswelwitschiaeand 15 strains ofA. niger.We have found new criteria in the morphological identification ofA.welwitschiaeas its colony color was black to brown with yellow edge, but inA. nigerwas black with white edge, alsoA.welwitschiaesometimesproducedfinely-to-distinctly roughened brownish conidia on malt extract agar (MEA) media. Thirteenisolates ofA.welwitschiaeand six ofA. nigerwere recognizedas potential producers for ochratoxin A.
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