Abstract
New carbohydrate-conjugate heterobimetallic complexes [C22H50N6O13CuSnCl2] (3) and [C22H58N6O17NiSnCl2] (4) were synthesized from their monometallic analogs [C22H52N6O13Cu] (1) and [C22H60N6O17Ni] (2) containing N-glycoside ligand (L). In vitro DNA binding studies of L and complexes (1–4) with CT DNA were carried out by employing various biophysical and molecular docking techniques which revealed that heterobimetallic complex 3 strongly binds to DNA in comparison to 4, monometallic complexes (1 and 2) and the free ligand. Complex 3 cleaves pBR322 DNA via hydrolytic pathway (confirmed by T4 DNA ligase assay) and inhibited Topo-II activity in a dose-dependent manner. Furthermore, complex 3 was docked into the ATPase domain of human-Topo-II in order to probe the possible mechanism of inhibition.
Published Version
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