Abstract
In this study; validation and optimization study of 6 new miniSTR was made to use these loci on forensic laboratory in Turkey. Coble and Butler's method [3] was based in this study but several modifications were made on this PCR method. Primer dye set should be FAM-TET-HEX rather than FAM-VIC-NED. Primer concentrations were increased. Top Taq™ Master Mix Kit (Qiagen) was used for PCR rather than use of single by single PCR solution. 6 loci were studied as two triple multiplex sets. After the method was optimized with these conditions, validation studies were carried out by working the sensitivity and reproducibility of this procedure and analyzing mixed samples.
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