Abstract
Purpose of the study. To study experimental model of purulent wounds efficiency of the combined effects of benzalkonium chloride and metronidazole immobilized on various bases.Materials and methods. The material was in vitro studies and experimental animals (Wistar rats), which was modeled a purulent wound and then divided into 3 groups according to the treatment method with the three studied combinations, each of which contained benzalkonium chloride and metronidazole, the combinations differed in bases, which were polyethylene oxide or sodium salt of carboxymethylcellulose, or polymethylsiloxane polyhydrate. Research methods: in an in vitro experiment, growth retardation zones were determined for the most common pathogens of wound infection using the standard disc method. In an in vivo experiment the healing process was evaluated by Popov's planimetric method, the contamination of the wounds was studied by sowing the biopsy of wounds on nutrient media with subsequent counting of the grown up colonies. The data were processed statistically, the reliability of the differences was assessed by the Kruskal-Wallis test.Results. All test combinations showed high growth retardation areas for all test strains of microorganisms. Objectively, the highest results were observed with a combination of polyethylene oxide (Staphylococcus aureus 30 (29; 30), Bacillus cereus 27 (27; 28), Escherichia coli 20.5 (20; 21), Proteus vulgaris 21.5 (20; 22), Pseudomonas aeruginosa 17 (16; 17), Candida albicans 25.5 (25; 26)). In determining the healing rate, it was noted that the maximum values in the groups when using combinations based on the sodium salt of carboxymethylcellulose and polymethylsiloxane polyhydrate occurred on day 1–3 (29.6 (27.6; 31) and 20.2 (18.8; 24.2) mm2/day, respectively), and in the group using a combination on polyethylene oxide — for 3–5 days (20.6 (17.3; 22.8) mm2/day).Conclusion. Our studies have shown the high antimicrobial activity of all the developed combinations for all the strains of microorganisms studied. It is also possible to recommend combinations on polyethylene oxide and sodium salt of carboxymethylcellulose for further preclinical studies at all times of treatment, and a combination based on polymethylsiloxane polyhydrate only in the first phase of the wound process.
Highlights
Our studies have shown the high antimicrobial activity of all the developed combinations for all the strains of microorganisms studied
Данные настоящего исследова‐ ния можно применять для дальнейших изысканий и доклинических испытаний в области местного лечения гнойных ран
Summary
ФГБОУ ВО «Курский государственный медицинский университет» Министерства здравоохранения Российской Федерации, 305041, Российская Федерация, г. Материалом явились исследования in vitro и экспериментальные животные (крысы по‐ роды Вистар), которым моделировалась гнойная рана с последующим разделением на 3 группы в соответствии со способом лечения тремя исследуемыми комбинациями, каждая из которых содержала бензалкония хлорид и метронидазол, отличались комбинации основами, коими явились полиэтиленоксид или натриевая соль карб‐ оксиметилцеллюлозы, или полиметилсилоксана полигидрат. Методы исследования: в эксперименте in vitro определялись зоны задержки роста в отношении наиболее распространенных возбудителей раневой инфекции методом стандартных дисков. Все исследуемые комбинации показали высокие зоны задержки роста в отношении всех иссле‐ дуемых тест-штаммов микроорганизмов. Также можно рекомен‐ довать комбинации на полиэтиленоксиде и натриевой соли карбоксиметилцеллюлозы для дальнейших докли‐ нических исследований на всех сроках лечения, а комбинацию на основе полиметилсилоксана полигидрата — лишь в первую фазу раневого процесса. Получено 15.11.2019, Рецензия (1) 13.03.2020, Рецензия (2) 20.04.2020, Принята к печати 24.06.2020
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