Abstract
New analytical techniques were used to study the kinetic behavior of 5-(hydroxmethyl) furfural (HMF) and blockage of available lysine in milk and model systems. Both determination of HMF by the chromatographic method and estimation of available lysine by the ortho-phthaldialdehyde (OPA) method at sterilization temperatures proved valid. Activation energies for the lysine loss reaction were 91.14, 112.41 and 66.67 kJ·mol−1 in the model systems and the milk respectively in fluorimetric determination with OPA. Activation energy for HMF determination ranged from 118.5 to 93.04 kJ·mol−1
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