New method of injecting solution into the abdomen to overcome fixation delay of the midgut on making the histological specimens of honeybee worker

Abstract

Few histological studies have been performed to evaluate infectious diseases in honeybees, as their water-resistant exoskeletons make the preparation of whole-body specimens difficult by blocking the permeation of fixative and substitution solutions. Additionally, it remains difficult to fix samples when specimens are collected far from the laboratory, as delays in fixation lead to the autolysis of abdominal organs. In this study, we propose an intraperitoneal fixative injection method to prevent fixation delay and a method for preparing honeybee tissue specimens for histopathological examinations.