Abstract
Nucleotides were separated on thin layer of PEI or standard cellulose developed in buffered aqueous ammonium isobutyrate at pH 4.6. In addition to R f values, nucleoside mono-, di-, and triphosphates were further differentiated according to the rate at which a nucleotide forms a stable blue complex with ammonium molybdate. The blue color formation, in turn, depends upon the ease at which a nucleotide liberates its P i; this property appears to be closely related to the nature of the phosphate-phosphate bond, the sugar-phosphate bond, and the base-sugar bond. Differentiation was made between: ( a) ribonucleotide and deoxyribo-nucleotide, ( b) purine derivative and pyrimidine derivative, ( c) 5′-ribonucleotide and 2′,3′-ribonucleotide, ( d) nucleoside monophosphates (e.g., 5′-AMP, 5′-CMP, 5′-UMP, and 5′-GMP), and ( e) nucleoside mono-, di-, and triphosphates (e.g., AMP, ADP, and ATP).
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