Abstract
New method was developed for separation and purification of polyunsaturated fatty acid ethyl esters using a silver ion-exchanged spherical clay mineral [SC (Ag)]. The SC (Ag) was prepared by ion-exchanging silver ion into synthetic hectorite that had been spray-dried to form a spherical shape. Ethyl icosapentaenoate and ethyl docosahexaenoate were separated and purified from the mixture of fatty acid ethyl esters originated from several natural lipids, using SC (Ag) preparative columns under the step gradient condition of organic solvent.
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