Abstract
Anew method was developed for assessing the changes in lumenal area in microexplants of airways caused by pharmacologic and physiologic activation. Microairway rings were sectioned from either 18 explanted guinea pig tracheal airways or 5 human bronchial airways and placed in microchambers containing 250 μL Hanks’ balanced salt solution. Tissues were focused through a stereozoom microscope, captured by video digitizing board, and transmitted continuously to the input of a video recorder. Changes in airway lumenal area were assessed as changes in pixel number by image analysis software and normalized as percent area reduction. As for isometrically fixed airway in large perfusate volume, microsection of airways caused a time-related spontaneous active tone.
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