New metabolizable immunologic adjuvant for human use. 6. Disposition of radioactivity after administration of labelled vaccine to the rat.

Abstract

SummaryThree batches of Adjuvant 65 were prepared, each labelled with C14 in one of the components, and emulsified with aqueous suspensions of killed influenza virus. The resulting vaccines were administered intramuscularly to rats, and the disposition of the tracer was followed for 120 days. The C14 from aluminum monostearate-1-C14 and from Arlacel A (mannide-l-C14-monooleate) disappeared from the injection site more rapidly than C14 from peanut oil (oil containing a small excess of glyceryltrioleate added as carboxyl C14-labelled triglyceride). Expiration of C14O2 by the rats which were injected with labelled aluminum stearate or labelled peanut oil vaccines demonstrates that these fatty materials reached the metabolic pools and were metabolized normally. Radioactivity injected with the vaccine as mannide-labelled Arlacel A did not accumulate in the tissues, most of it being excreted via the urine. It is concluded that the fatty components of the Adjuvant 65 vaccines were absorbed after intramuscular ad...