Abstract
Four new compounds, including two isocoumarins, pestaloisocoumarins A and B (1, 2), one sesquiterpenoid degradation, isopolisin B (4), and one furan derivative, pestalotiol A (5), together with one known isocoumarin, gamahorin (3), and three chlorinated benzophenone derivatives, pestalachloride B (6), pestalachloride E (7) and a mixture of pestalalactone atropisomers (8a/8b), were isolated from a culture of the fungus Pestalotiopsis heterocornis associated with sponge Phakellia fusca. These new chemical structures were established using NMR and MS spectroscopic data, as well as single-crystal X-ray crystallographic analysis and CD Cotton effects. All of the isolated compounds were evaluated for their antimicrobial and cytotoxic activities. Isocoumarins 1–3, showed antibacterial activities against Gram-positive bacteria Staphylococcus aureus and Bacillus subtilis with MIC values ranging from 25 to 100 μg/mL and weak antifungal activities. Chlorinated benzophenone derivatives 6–8 exhibited antibacterial activities against S. aureus and B. subtilis with MIC values ranging from 3.0 to 50 μg/mL and cytotoxicities against four human cancer cell lines with IC50 values of 6.8–87.8 μM.
Highlights
Fungi of the genus Pestalotiopsis are widely distributed throughout the world and have proven to be a rich source of bioactive natural products [1]
With of thea aim of discovering substances from marine-derived fungi, the secondary metabolites culture fermentationbioactive of Pestalotiopsis heterocornis, marine‐derived fungi, the secondary metabolites of a culture fermentation of Pestalotiopsis heterocornis, which was isolated from the sponge Phakellia fusca, were investigated
Compounds 6–8 were inactive against three test isocoumarins 1–3 exhibited weak antifungal activities against three test fungi or part of them fungi at 100 μg/mL, and Compounds 4, 5 did not show antimicrobial activity against any test with minimum inhibitory concentration (MIC) values 100 μg/mL
Summary
Fungi of the genus Pestalotiopsis are widely distributed throughout the world and have proven to be a rich source of bioactive natural products [1]. Marine fungi of the genus Pestalotiopsis associated with the sponges could to metabolize biologically interesting and chemically diverse compounds. With of thea aim of discovering substances from marine-derived fungi, the secondary metabolites culture fermentationbioactive of Pestalotiopsis heterocornis, marine‐derived fungi, the secondary metabolites of a culture fermentation of Pestalotiopsis heterocornis, which was isolated from the sponge Phakellia fusca, were investigated. Cotton effects structures of the new compounds were elucidated on the basis of spectroscopic data, circular anddichroism single-crystal analysis.X‐ray.
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