Abstract
BackgroundBioprospecting lipase producers in non-conventional habitats are the way to find special enzymes of diverse applications. Halo-alkaline marshes in Wadi El Natrun in Egypt are some of the most stable ecological systems in the world, and because of the double extremities of alkalinity and salinity, they harbor individual microbes capable of adapting stress conditions.ResultsEight strains were recovered from the coastline soil of Al-Beida Lake in Wadi El Natrun and have been tested for lipase production. Among the eight isolates, the strain SBLWN_MH2 was the most active producer of lipase (7.5 U/ml). The crude SBLWN_MH2 lipase showed activity over a wide pH range (3.5 to 13) with an optimum pH at 10.5, and it was able to show more than 75% of its highest activity at pH elevated up to 13. The identification using phenotypic and genotypic methods strongly indicated that the strain SBLWN_MH2 belonged to the genus Streptomyces with a similarity of 99%. Thus, it has been given the suggested name Streptomyces sp. SBLWN_MH2 (MG593538). SBLWN_MH2 produced extracellular lipase in modified starch casein medium supplemented with different oils or Tween-80, and the potential production rate has been attained in the case of linseed oil after 3 days. Further experiments have been carried out to optimize medium composition through Box-Behnken design and response surface methodology, and it was possible to achieve more than 3.5-fold increase in lipase production.ConclusionsThe present study indicates that Streptomyces sp. SBLWN_MH2 is a potential lipase producer and could be fruitfully employed in the large-scale production of highly alkaline lipase.
Highlights
Bioprospecting lipase producers in non-conventional habitats are the way to find special enzymes of diverse applications
Salihu and Alam [13] reviewed many bacterial genera that were capable of producing alkaline lipases, and there was a scarce documentation for strains isolated from halo-alkaline habitats
3.1 Isolation of Streptomyces producing lipase Isolation plates from samples collected at latitude and longitude of 30° 26′ 20′′ N and 30° 14′ 44.0′′ E showed repeated occurrence of eight different strains of Streptomyces, and so those strains were further streaked on the surface of agar plates before being transferred to slants of the same isolation medium
Summary
Supakdamrongkul et al [39] found that castor oil potentiated the highest yield of lipase by the fungus Nomuraea rileyi, while the linseed oil induced the lowest production yield Along these lines, the hydrolytic activity profile of various lipases against various substrates ought to be utilized as a basic illustrative character in enzyme production. The advantage of the Box-Behnken design in profiling interaction between variables was associated with the further advantage of defining the parameters for attaining 26.5416 U/ml of lipase (more than 3.5-fold increase in initial yield of 7.5 U/ml) which is more suitable for bioreactors studies
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