New intragastric sleeve technique reduces adipose tissue in pig experimental model: tomographic study

Mariana Da Silva Ribeiro,Saulo José Quina Silva,Ricardo Paiva Araújo Dos Scheiba Zorron,Celia Raquel Quirino,Fernanda Antunes,Marcelo Borges Dos Santos Junior,Silvia Marcela Ruiz Cadena,Guilherme De Souza Vieira,André Lacerda De Abreu Oliveira

doi:10.1038/s41598-020-74846-8

Mariana Da Silva Ribeiro, Saulo José Quina Silva + Show 7 more

Open Access

https://doi.org/10.1038/s41598-020-74846-8

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Journal: Scientific Reports	Publication Date: Oct 22, 2020
License type: open-access

Affiliation: Klinikum Bremerhaven-Reinkenheide

Abstract

In order to implement a new bariatric surgery technique, we verify the efficacy of intragastric sleeve to reduce weight gain and subcutaneous adipose tissue (SAT). Animals were divided into two groups: G1 (single-port intragastric sleeve) and G2 (sham group). The stomach was surgically reduced by single-port intragastric sutures to fo a gastric sleeve. Animals were submitted to computer tomography (CT) before the surgical procedure and after 18 weeks.Images were analyzed and measurements of the thickness of SAT, depth and width of the longissimus dorsi muscle and the rib eye area were made. Body weight and CT measurements were analyzed using the GLM PROC. The correlation coefficients were calculated among weight, moments and measures. There was a significant difference in weight gain, in which G1 had an average of 42.803 ± 3.206 kg, lower than G2 (45.966 ± 4.767 kg). The mean values for SAT and muscle measurements differed significantly between groups, in which G1 achieved the lowest values. All variables had significant correlations and high magnitude. Intragastric sleeve surgery induced a significant decrease of SAT. The new intragastric sleeve technique is feasible, safe and effective, mainly in reducing fat deposition, making it an important alternative in bariatric surgical treatment.

Highlights

MethodsThe research was approved by the Committee on Ethical Use of Animals of The State University of Northern Rio de Janeiro (protocol 765311) and were in accordance with the ethical standards of the institution
In order to implement a new bariatric surgery technique, we verify the efficacy of intragastric sleeve to reduce weight gain and subcutaneous adipose tissue (SAT)
Animals were divided into experimental group (G1), which was submitted to single-port intragastric sleeve surgery by endoplication (IGS-IGP); and the sham group (G2), submitted to celiotomy followed by gastrostomy

Summary

Methods

The research was approved by the Committee on Ethical Use of Animals of The State University of Northern Rio de Janeiro (protocol 765311) and were in accordance with the ethical standards of the institution. Animals were divided into experimental group (G1), which was submitted to single-port intragastric sleeve surgery by endoplication (IGS-IGP); and the sham group (G2), submitted to celiotomy followed by gastrostomy. Animals were submitted to CT (GE LightSpeed PRO 32-slice scanner) and were placed in sternum decubitus with anterior limbs stretched above their heads to evaluate subcutaneous adipose tissue thickness (SAT), width (WLM) and depth (DLM) of the longissimus dorsis muscle, and loin eye area (LEA), measured above the last rib. This evaluation was performed in two moments: one day before surgery and on the day of death of the animals. This article does not contain any studies with human participants performed by any of the authors

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Conclusion