Abstract
The research community is generally agreed that maintenance of healthy levels of free radicals and related oxidants are important for good health. However, utilization of the “redox stress hypothesis” can provide us with concrete nutritional targets in order to better support and maintain “optimal health.” Following this hypothesis we performed a crossover, double-blind, placebo-controlled, single-dose study on the effects of SPECTRA™, a dietary supplement, on oxidative stress markers (OSM) in human participants (n = 22). The measurement of OSM (ex vivo intra- and extracellular formation of reactive oxygen species (ROS, O2−, H2O2, OH−) in whole blood, respiratory activity of blood cells, as well as mitochondrial-dependent ROS formation, and respiratory activity), was performed using EPR spectrometer nOxyscan, spin probe CMH, and oxygen label NOX-15.1, respectively. Furthermore, we investigated the ability of SPECTRA™ to modulate ex vivo cellular inflammatory responses induced by stimulation with exogenous TNF-α and also followed changes in bioavailable NO concentrations. In this clinical study, we demonstrated that administration of SPECTRA™ resulted in statistically significant long-term inhibition of mitochondrial and cellular ROS generation by as much as 17% as well as 3.5-times inhibition in extracellular NADPH system-dependent generation of O2−, and nearly complete inhibition of extracellular H2O2 formation. This was reflected in more than two times inhibition of ex vivo cellular inflammatory response and also increases in bioavailable NO concentration. For the first time, we have measured synergetic, biological effects of a natural supplement on changes in OSM and cellular metabolic activity. The unique design and activity of the plant-based natural supplement, in combination with the newly developed and extended Vitality test, demonstrates the potential of using dietary supplements to modulate OSM and also opens the door to future research into the use of natural supplements for supporting optimal health.
Highlights
During the last four decades, the research community has generally agreed that a dynamic, appropriately reactive, and healthy balance between levels of free radicals and levels of related oxidants is important for “optimal health.” Imbalances of free radicals, and potentially unhealthy levels of oxidants versus antioxidants, are collectively defined by the scientific community as “oxidative and nitrosative stress.” The delicate balance between beneficial and harmful effects caused by reactive oxygen and nitrogen species (RONS) is an important aspect of living organisms
Food Science & Nutrition published by Wiley Periodicals, Inc
Noxygen Science Transfer & Diagnostics GmbH (Elzach, Germany) designed a bench-top Electron paramagnetic resonance (EPR) spectrometer “nOxyscan.” These advances in instrumentation provided us with an opportunity to perform a pilot study to investigate the bioactivity of a nutritional supplement SPECTRATM, a formulation consisting of high antioxidant activity fruit, vegetable concentrates, and herbal extracts, manufactured by FutureCeuticals, Inc. (Momence, IL) and standardized to a minimum of total antioxidant capacity (TAC) as measured by a series of oxygen radical absorbance capacity (ORAC)—based assays collected under the name ORAC 5.0, including ORAC, HORAC, NORAC, SORAC, and SOAC (Mullen et al 2011)
Summary
During the last four decades, the research community has generally agreed that a dynamic, appropriately reactive, and healthy balance between levels of free radicals and levels of related oxidants is important for “optimal health.” Imbalances of free radicals, and potentially unhealthy levels of oxidants versus antioxidants, are collectively defined by the scientific community as “oxidative and nitrosative stress.” The delicate balance between beneficial and harmful effects caused by reactive oxygen and nitrogen species (RONS) is an important aspect of living organisms. New Insights on Effects of a Dietary Supplement new view, aging is the result of functional losses that are primarily caused by a progressive pro-oxidizing shift in the redox status of cells and tissues. The effect of reactive oxygen species (ROS) that may cause potential biological damage has been termed “oxidative stress” and the effect of reactive nitrogen species (RNS) has been termed “nitrosative stress” (Kovacic and Jacintho 2001; Valko et al 2001; Ridnour et al 2005) This model presents us with concrete targets for potential nutritional intervention in order to maintain optimal health and support healthy aging and provides a means to investigate the direct effects of nutritional materials on biomarkers of significance for healthy aging (Broedbaek et al 2013). Noxygen Science Transfer & Diagnostics GmbH (Elzach, Germany) designed a bench-top EPR spectrometer “nOxyscan.” These advances in instrumentation provided us with an opportunity to perform a pilot study to investigate the bioactivity of a nutritional supplement SPECTRATM, a formulation consisting of high antioxidant activity fruit, vegetable concentrates, and herbal extracts, manufactured by FutureCeuticals, Inc. (Momence, IL) and standardized to a minimum of total antioxidant capacity (TAC) as measured by a series of oxygen radical absorbance capacity (ORAC)—based assays collected under the name ORAC 5.0, including ORAC, HORAC, NORAC, SORAC, and SOAC (Mullen et al 2011)
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