New insights of cyanoacrylate glues in surgical applications. A comparative study of akyl cyanoacrylates.

Abstract

Event Abstract Back to Event New insights of cyanoacrylate glues in surgical applications. A comparative study of akyl cyanoacrylates. Julio San Román1, Mar Fernandez-Gutierrez1, Gemma Pascual2, Barbara Perez-Kholer2 and Juan M Bellon2 1 Institute of Polymers and CIBER-BBN, Biomaterials, Spain 2 University of Alcala de Henares and CIBER-BBN, Faculty of Medicine, Spain Alkyl cyanoacrylates have been applied since 1965 as self-adhesives for biomedical applications because of their good properties and very fast curing process in physiological conditions, associated to the ionic polymerization catalysed by water. However the stability of the poly(alkyl cyanoacrylate)s after the application in contact with living tissues depends on the chemical structure of the corresponding alkyl cyanoacrylates and poly(alkyl cyanoacrylates) formed by the polymerization reaction, being the reaction of the ethyl derivative faster than butyl, hexyl or longer alkyl segments. In addition, the toxicity of these systems is also associated to the nature of the alkyl residue, being the ethyl cyanoacrylate much more toxic that the butyl, hexyl or longer hydrocarbon substituents. The aim of the present work is the comparative analysis in vitro and in vivo of two commercial akyl cyanoacrylate monomers, Glubran 2 (n-butyl cyanoacrylate) and Ifabond (n-hexyl cyanoacrylate) in comparison with an experimental bioadhesive based on n-octyl cyanoacrylate, different from Dermabond (2-octyl cyanoacrylate) indicated for topical applications in dermal defects. The three systems were tested “in vitro” with different cell lines , and “in vivo” by the fixation of surgical meshes of polypropylene applied to abdominal hernias repair, using rabbits as experimental animal model and a methodology previously validated by the group. Adhesion of the mesh by the application of the three adhesives and comparison with suture, fibrosis and tissue integration as well as inflammation and local effects of the three adhesives were determined after periods post-implantation of 14 , 30 and long term 90 days. In addition the biomechanical properties of the meshes integrated into the abdominal wall of the rabbit were determined according to a well-defined protocol using strips of the integrated meshes prepared from the implants. The results demonstrated that the Glubrand and Ifabond glues give a more inflammatory response than the n-octyl cyanoacrylate after the study of the histological sections of the points of application and the biomechanical behaviour is very good for the octyl derivative even after the 90 days of implantation. The resorption of the polymer generated by the systems is also dependent of the nature of the adhesives, being the n-octyl derivative more stable than the commercial glues. The “in vitro” test based on standard analysis MTT and Alamar Blue, indicate a good tolerance and biocompatibility of the three alkyl cyanoacrylates studied, but it is clear that the viability of n-octyl cyanoacrylate is better than the other commercial glues, Glubrand 2 and Ifabond. A detailed study of the cell behaviour and histological evaluation of the implants will be presented. projects SAF2014-55022-P and MAT2014-51918-C2-1R Keywords: Tissue Engineering, Scaffold, in vivo tissue engineering, bioactive interface Conference: 10th World Biomaterials Congress, Montréal, Canada, 17 May - 22 May, 2016. Presentation Type: Poster Topic: Surface and interfacial characterization Citation: San Román J, Fernandez-Gutierrez M, Pascual G, Perez-Kholer B and Bellon J (2016). New insights of cyanoacrylate glues in surgical applications. A comparative study of akyl cyanoacrylates.. Front. Bioeng. Biotechnol. Conference Abstract: 10th World Biomaterials Congress. doi: 10.3389/conf.FBIOE.2016.01.02828 Copyright: The abstracts in this collection have not been subject to any Frontiers peer review or checks, and are not endorsed by Frontiers. They are made available through the Frontiers publishing platform as a service to conference organizers and presenters. The copyright in the individual abstracts is owned by the author of each abstract or his/her employer unless otherwise stated. Each abstract, as well as the collection of abstracts, are published under a Creative Commons CC-BY 4.0 (attribution) licence (https://creativecommons.org/licenses/by/4.0/) and may thus be reproduced, translated, adapted and be the subject of derivative works provided the authors and Frontiers are attributed. For Frontiers’ terms and conditions please see https://www.frontiersin.org/legal/terms-and-conditions. Received: 27 Mar 2016; Published Online: 30 Mar 2016. Login Required This action requires you to be registered with Frontiers and logged in. To register or login click here. Abstract Info Abstract The Authors in Frontiers Julio San Román Mar Fernandez-Gutierrez Gemma Pascual Barbara Perez-Kholer Juan M Bellon Google Julio San Román Mar Fernandez-Gutierrez Gemma Pascual Barbara Perez-Kholer Juan M Bellon Google Scholar Julio San Román Mar Fernandez-Gutierrez Gemma Pascual Barbara Perez-Kholer Juan M Bellon PubMed Julio San Román Mar Fernandez-Gutierrez Gemma Pascual Barbara Perez-Kholer Juan M Bellon Related Article in Frontiers Google Scholar PubMed Abstract Close Back to top Javascript is disabled. Please enable Javascript in your browser settings in order to see all the content on this page.