Abstract
Agriculture is a major anthropogenic source of the greenhouse gas N2O, which is also involved in stratospheric ozone depletion. While the use of rhizobial inoculants has already been reported as an emerging option for mitigating soil N2O emissions, this study presents an in situ abatement of 70% of soil N2O emission using the strain nosZ+ G49 vs. nosZ− USDA138 in association with soybean. Therefore, we consider that the choice of the inoculant strain of a leguminous crop should take into account the capacity of strains to reduce nitrous oxide in addition to their N fixation capacity. This study also clearly suggests that this mitigation option could be considered not only for soybean but also for different leguminous crops, with emphasis currently placed on lupin because of the potential of its association with the nosZ+ LL200 strain. The clear demonstration of the N2O reduction capacity of clover symbiotic strains suggests that opportunities for mitigation might also occur on grassland.
Highlights
The two sets of primers used gave fully correlated results concerning the detection or the absence of detection of the nosZ gene, and the results obtained with the reference strains, B. diazoefficiens strain USDA110 and Ensifer meliloti strain 2011, were consistently positive
Sequencing of several PCR products confirmed that amplification products corresponded to nosZ sequences
While the use of rhizobial inoculants to mitigate N2 O emissions has already been presented as an emerging option for mitigating N2 O emissions from food production, this study presents an in situ proof of concept of this phenomenon in a soybean crop, by demonstrating an N2 O emission abatement of 70% when using the nosZ+ G49 strain compared to the nosZ− USDA138 strain, in association with soybean
Summary
New. Agriculture, through soil emissions, is a major anthropogenic source of the greenhouse gas N2 O, which has a Global Warming Potential about 300 times higher than CO2 on a molar basis, and which is involved in stratospheric ozone depletion [1]. N2 O is mainly produced through the microbial processes of denitrification and nitrification [2]. The last step of the denitrification process, N2 O reduction into N2 , is currently the only known pathway for the terrestrial removal of N2 O. N2 O reduction is catalyzed by the. Strategies to mitigate N2 O emissions from agricultural soil could be based on promoting the reduction of N2 O into N2 in soils [3]. Bakken and Frostegård [4] reviewed emerging options for mitigating
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