Abstract
Bovine intramammary infections are common diseases affecting dairy cattle worldwide and represent a major focus of veterinary research due to financial losses and food safety concerns. The identification of new biomarkers of intramammary infection, useful for monitoring the health of dairy cows and wellness verification, represents a key advancement having potential beneficial effects on public health. In vitro experiments using bovine peripheral blood mononuclear cells (PBMC), stimulated with the bacterial endotoxin lipopolysaccharide (LPS) enabled a flow cytometric assay in order to evaluate in vivo poly-ADP-ribose (PAR) levels. Results showed a significant increase of PAR after 1 h of treatment, which is consistent with the involvement of PARP activity in the inflammatory response. This study investigated PARP-1 activation in leukocyte subpopulations from bovine milk samples during udder infection. A flow cytometric assay was, therefore, performed to evaluate the PAR content in milk leukocyte subsets of cows with and without intramammary infection (IMI). Results showed that milk lymphocytes and macrophages isolated from cows with IMI had a significant increase of PAR content compared to uninfected samples. These results suggest mastitis as a new model for the study of the role of PARP in zoonotic inflammatory diseases, opening a new perspective to the “One Health” approach.
Highlights
Published: 9 March 2021The global health of humans and animals is interconnected, indicating the need for a “One Health” perspective
Side Scatter (SSC) does not distinguish between the principal cell populations present in milk, including lymphocytes, neutrophils, and macrophages, which play an important role in the defense of mammary gland against invading pathogens and in the resolution of infectious disease [2]
We showed that increased Somatic cell count (SCC) is associated with PARP-1 activation during bovine mastitis
Summary
The global health of humans and animals is interconnected, indicating the need for a “One Health” perspective. Infectious diseases that are naturally transmitted from animals to humans, and antimicrobial resistance are recognized as threats to global health and pose a major public health concern. Food is one of the most important vehicles for the transmission of resistant bacteria from animals to humans, and the consumption of food containing antibiotic-resistant bacteria has led to the acquisition of antimicrobial-resistant infections. SSC does not distinguish between the principal cell populations present in milk, including lymphocytes, neutrophils, and macrophages, which play an important role in the defense of mammary gland against invading pathogens and in the resolution of infectious disease [2]
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