New Insights Into the Role of Follicle-Stimulating Hormone in Sex Differentiation of the Protogynous Orange-Spotted Grouper, Epinephelus coioides.

Minwei Huang,Huimin Chen,Yong Zhang,Zeshu Yu,Haoran Lin,Yun Liu,Zhifeng Ye,Ling Xiao,Cheng Peng,Mi Zhao,Shuisheng Li,Jiaxing Chen

doi:10.3389/fendo.2019.00304

Abstract

Follicle-stimulating hormone (FSH) signaling is considered to be essential for early gametogenesis in teleosts, but its functional roles during sex differentiation are largely unknown. In this study, we investigated the effects of long-term and short-term FSH injection on sex differentiation in the protogynous orange-spotted grouper (Epinephelus coioides). Long-term FSH treatment initially promoted the formation of ovaries but subsequently induced a male fate. The expression of female pathway genes was initially increased but then decreased, whereas the expression of male pathway genes was up-regulated only during long-term FSH treatment. The genes related to the synthesis of sex steroid hormones, as well as serum 11-ketotestosterone and estradiol, were also up-regulated during long-term FSH treatment. Short-term FSH treatment activated genes in the female pathway (especially cyp19a1a) at low doses but caused inhibition at high doses. Genes in the male pathway were up-regulated by high concentrations of FSH over the short term. Finally, we found that low, but not high, concentrations of FSH treatment activated cyp19a1a promoter activities in human embryonic kidney (HEK) 293 cells. Overall, our data suggested that FSH may induce ovarian differentiation or a change to a male sex fate in the protogynous orange-spotted grouper, and that these processes occurred in an FSH concentration-dependent manner.

Highlights

It is well-established that sex steroid hormones play a crucial role during gonadal differentiation in teleost fish [1,2,3]
We discovered that long-term Follicle-stimulating hormone (FSH) treatment firstly accelerates ovarian differentiation but subsequently induces female-to-male sex reversal in orange-spotted grouper during sex differentiation
The expression of fshβ was low in females but increased significantly during female-to-male sex reversal in the honeycomb grouper

Summary

Introduction

It is well-established that sex steroid hormones play a crucial role during gonadal differentiation in teleost fish [1,2,3]. The administration of exogenous steroids in undifferentiated fish can direct the process of sexual differentiation toward a specific sex fate. Treatments with exogenous androgens, or an aromatase inhibitor (AI), can induce a male fate in some fish, regardless of their genetic sex [4,5,6]. Genetic males can be driven into a female fate by estrogen [7, 8]. The balance of endogenous androgens and estrogens play a crucial role during sex differentiation. The upstream mechanisms underlying the synthesis of gonadal sex steroid hormones during sex differentiation are largely unknown

Methods

Results

Conclusion