Abstract
Extracellular polymer proteins have been reported to play an important role in enhancing sludge dewaterability during anaerobic digestion in our previous study. However, how the proteins in sludge determine sludge dewaterability remains to be determined. In this work, proteins from digested sludge were identified using label free proteomics analysis, and its hydrophilicity/hydrophobicity properties and functional groups were analysed. We determined that the microbial community variation between the three stages during the anaerobic digestion process was responsible for enhancing sludge dewaterability; The transformation from hydrophilicity to hydrophobicity of digested sludge surface is the result of functional groups distribution variation which caused by the proteins and microbial communities. This study provides a new insight into the development of anaerobic digestion based on sludge dewaterability.
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