Abstract
The medicinal plant Spathodea campanulata P. Beauv. (Bignoniaceae) has been traditionally applied for the prevention and treatment of diseases of the kidney and urinary system, the skin, the gastrointestinal tract, and inflammation in general. The present work shows for the first time how chemical components from this plant inhibit Helicobacter pylori growth by urease inhibition and modulation of virulence factors. The crude extract and the main fractions of S. campanulata bark were tested on H. pylori isolated strains and the active ones were further fractionated. Fractions and sub-fractions of the plant crude extract were characterized by ultra-high-performance liquid chromatographic tandem high resolution-mass spectrometry detection (UHPLC-HRMS). Several phenolics and triterpenoids were identified. Among the sub-fractions obtained, SB2 showed the capacity to inhibit H. pylori urease in a heterologous bacterial model. One additional sub-fraction (SE3) was able to simultaneously modulate the expression of two adhesins (HopZ and BabA) and one cytotoxin (CagA). The flavonol kaempferol was identified as the most interesting compound that deserves further investigation as a new hit for its capacity to modulate H. pylori virulence factors.
Highlights
Helicobacter pylori (H. pylori) is a Gram-negative bacterium that persistently colonizes the stomach of more than half the human population and its prevalence is significantly higher in the developing countries [1,2]
To identify new anti-H. pylori growth inhibitors, we first tested fractions and sub-fractions of bark of S. campanulata against the bacterium and we focused on the urease enzyme and adhesin proteins involved in adhesion to gastric mucosa
As for the other two sub-fractions some virulence factors and by modulating the expression of the adhesins blood group antigen-binding adhesion (BabA) and HopZSD1 andand the SF, their chemical composition was not disclosed by cytotoxin CagA. These results suggest that enriched extracts from this plant could be useful for the Phenolics have general antimicrobial activity and and they somecould of them active against treatment of H
Summary
Helicobacter pylori (H. pylori) is a Gram-negative bacterium that persistently colonizes the stomach of more than half the human population and its prevalence is significantly higher in the developing countries [1,2]. It causes gastric inflammation, gastric mucosa-associated lymphoid tissue lymphoma, and cancer. Several studies have described high resistance to antibiotic treatment [5,6,7]. In 2017 WHO included H. pylori in the list of antibiotic resistant bacterium for which the identification and development of new antimicrobial drugs represent a global priority [8]
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a call
