Abstract
This study aimed to estimate the effects of increasing doses of Allium ursinum methanol extract on cardiac ischemia/reperfusion injury (I/R) with a special emphasis on the role of oxidative stress. Fifty rats were randomly divided into five groups (10 animals per group) depending on the applied treatment as follows: sham, rats who drank only tap water for 28 days and hearts were retrogradely perfused for 80 min without I/R injury, I/R, rats who drank only tap water for 28 days and hearts were exposed to ex vivo I/R injury and rats who consumed increasing doses of A. ursinum 125, 250, and 500 mg/kg for 28 days before I/R injury. Hearts from all rats were isolated and retrogradely perfused according to the Langendorff technique. Parameters of oxidative stress were spectrophotometrically measured in blood, coronary venous effluent, and heart tissue samples. Intake of wild garlic extract for 28 days significantly contributed to the recovery of cardiac function, which was reflected through preserved cardiac contractility, systolic function, and coronary vasodilatory response after ischemia. Also, wild garlic extract showed the potential to modulate the systemic redox balance and stood out as a powerful antioxidant. The highest dose led to the most efficient decrease in cardiac oxidative stress and improve recovery of myocardial function after I/R injury. We might conclude that wild garlic possesses a significant role in cardioprotection and strong antioxidant activity, which implicates the possibility of its use alone in the prevention or as adjuvant antioxidant therapy in cardiovascular diseases (CVD).
Highlights
Acute myocardial infarction (AMI) and heart failure (HF) that often complicates this condition are among the leading causes of death and disability worldwide (Hausenloy et al, 2019)
Minimum Rate of Left Ventricular Pressure Development Comparing the value of dp/dt min between groups treated with A. ursinum methanol extract (AUE) and rats from I/R and sham groups, it was observed a statistically significant increase in this parameter in point 7 in groups of rats treated with different doses of extract
Numerous preclinical (Kurian and Paddikkala, 2009, 2010; Kurian et al, 2010) and clinical studies have demonstrated that consumption of polyphenols, which are present in dietary plants such as A. ursinum is associated with cardiovascular risk reduction
Summary
Acute myocardial infarction (AMI) and heart failure (HF) that often complicates this condition are among the leading causes of death and disability worldwide (Hausenloy et al, 2019). If the disrupted balance between myocardial oxygen demand and supply persists for a long time, the tissue necrosis occurs leading to the formation of a non-contractile fibrotic scar that reduces myocardial contractile force (Curran et al, 2019; Kusmic and L’Abbate, 2020). Restoration of blood flow to ischemic tissue delivers blood-borne formed elements that in turn lead to increased oxidative stress by generating of ROS (reactive oxygen species) and bioavailability reduction of nitric oxide (NO) as an intracellular cardioprotective agent. These later cause exacerbation of parenchymal cell damage that induces secondary injury of tissue (Rout et al, 2020). The pathogenesis of I/R injury consists of many mechanisms, while the exact underlying molecular mechanisms of lethal reperfusion injury are not fully known and remain a huge challenge for clinicians (Neri et al, 2017)
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Free) 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a call
