Abstract
Brown rot is a worldwide fungal disease of stone and pome fruit that is caused by several Monilinia species. Among these, Monilinia fructicola can cause severe preharvest and postharvest losses, especially for stone fruit. Here, we present a high-quality draft genome assembly of M. fructicola Mfrc123 strain obtained using both Illumina and PacBio sequencing technologies. The genome assembly comprised 20 scaffolds, including 29 telomere sequences at both ends of 10 scaffolds, and at a single end of 9 scaffolds. The total length was 44.05 Mb, with a scaffold N50 of 2,592 kb. Annotation of the M. fructicola assembly identified a total of 12,118 genes and 13,749 proteins that were functionally annotated. This newly generated reference genome is expected to significantly contribute to comparative analysis of genome biology and evolution within Monilinia species.
Highlights
Monilinia fructicola is one of most important causal agents of brown rot, which is one of the main diseases of pome and stone fruit
Genome sequencing for short 2Â 100-bp paired-end reads (HiSeq 2500 platform; Illumina Sequencing Technology) and long 20-kb reads (RSII platform; Pacific Biosciences (PacBio) Sequencing Technology) were both performed by an external service (Macrogen Inc., Next-Generation Sequencing Service, Geumcheon-gu, Seoul, South Korea)
The number of large scaffolds reconstructed in the M. fructicola Mfrc123 strain corresponded to the 16 large chromosomes of the closely related species B. cinerea and Sclerotinia sclerotiorum (Amselem et al 2011; Derbyshire et al 2017)
Summary
Monilinia fructicola (phylum Ascomycota, family Sclerotiniaceae) is one of most important causal agents of brown rot, which is one of the main diseases of pome and stone fruit. Brown rot can cause severe yield losses during both field production and postharvest processing (Mari et al 2012; Karaca et al 2014; Oliveira Lino et al 2016; Abate, Pastore, et al 2018). Monilinia fructicola was introduced into Europe in 2001 (Lichou et al 2002), and rapidly spread and became prevalent over the former indigenous species M. laxa and M. fructigena (CABI 2018; Abate, Pastore, et al 2018). This fungus overwinters on mummified fruit or in infected plant tissues. The most severe yield losses occur in the postharvest phase, during fruit storage and transport (Feliziani et al 2013; Martini and Mari 2014)
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