Abstract
ABSTRACT Our aim was to screen new hyper-variable mango SSRs (MSSRs) designed from Amrapali genome sequences to find polymorphic ones between Amrapali and Sensation parental genotypes. Out of 253 MSSRs screened against Amrapali and Sensation genotypes, 212 were polymorphic, of which 8 MSSRs showed aa, bb or bb, aa allelic patterns. MSSRs showing polymorphism in terms of homozygous for ‘a’ allele in Amrapali and homozygous ‘b’ allele for Sensation and vice versa were selected for assignment of parentage of hand-pollinated (Amrapali x Sensation) hybrids and open-pollinated Amrapali. MSSR markers were mapped back to the Amrapali genome version 3.0 NCBI GenBank database. MSSRs (M109, P125, M15, and M73) assigned parentage to more than 88.0% of hand-pollinated hybrids. P125 confirmed parentage of 93.61% progenies followed by M109 (90.43%), M15 (89.36%), and M73 (88.29%). Randomly three MSSRs, viz. M109, P125, and M73, were used for assignment of parentage of OP seedlings of Amrapali. Out of 22 OP seedlings, only 2 seedlings showed the identical heterozygous allelic pattern and their hybrid origin was confirmed. It is concluded that MSSRs (P125, M109, M15, and M73) from the Amrapali genome have immense value in MAS in mango and can be utilised for early stage selection of true hybrids.
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