New Generation Expression Host System- Aiming high for commercial production of recombinant protein

Abstract

The global requirements of recombinant protein have increased exponentially in last one decade in various aspects. The commercial production of recombinant proteins requires an ideal host expression system which effectively meets the demand. Numerous host expression systems have been used to achieve level of expression. Escherichia coli BL21 (DE3) have been used ideally for expression of numbers of recombinant proteins from many decades. Though, Escherichia coli Bl21 is quite enough to express recombinant protein in higher fold but often get fails to achieve over expression of protein as essential requirement for today demand. Recently many engineered strains have been implemented in order to bring expression level as per requirement. The complications which arise in the expression of recombinant protein are mainly due to codon bias or inability of vector promoter to utilize host polymerase; while for over-expression of recombinant proteins often inhibit itself due to protein toxicity in conventional expression host systems. Escherichia coli C41 (DE3), Escherichia coli C43 (DE3) and Escherichia coli Rosetta are the refined and engineered strains which have shown over-expression of recombinant protein in recent time. These strains are designed to overcome the complication often limits conventional expression system.