New Gene Profiling in Determination of Breast Cancer Recurrence and Prognosis in Iranian Women

Seyed Mohammad Poorhosseini,Zeinab Ravesh,Hamid Reza Kheiri,Mohammad Hashemi,Feyzollah Hashemi-Gorji,Vahid Reza Yassaee,Nasrin Alipour Olyaei,Amir Izadi,Elham Moslemi

doi:10.7314/apjcp.2016.17.s3.155

Seyed Mohammad Poorhosseini, Zeinab Ravesh + Show 7 more

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https://doi.org/10.7314/apjcp.2016.17.s3.155

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Abstract

Breast cancer (BC) is the second most common cancer in the world and by far the most frequent cancer among women, with an estimated 1.67 million new cancer cases diagnosed in 2012 (25% of all cancers). Polygene expression analysis is used to predict the prognosis and determine the most appropriate treatment regimen. The objective of this study was to examine the gene expression profiles of SIRT3, HRAS, LSP1, SCUBE2 and AP2A2 in Iranian women with BC.A total of 136 patients including healthy controls were categorized into three groups based on the relapse of the disease. Expression of desired genes in formalin-fixed, paraffin embedded tissues collected from all groups of participants was analyzed via the RT PCR method. RNA extraction and cDNA synthesis were performed then real-time quantitative PCR was carried out. Gene expression analysis revealed that the expression of SIRT3 was equal among patient and control groups. LSP1 was down regulated in all patient groups relative to controls but reduced expression in the metastatic group relative to the non-metastatic one was not significant. HRAS was significantly overexpressed in total and metastatic tumor samples versus normal but not in non-metastatic cases. SCUBE2 expression showed significant over-expression in both overall tumor samples and the non-metastatic group as compared to normal tissues. Gene expression level of AP2A2 in all groups was not detectable. Our data are compatible with a tumor suppressor role of LSP1 related to potential prognostic factor for tumor recurrence and outcome. This study for the first time assayed the prognostic value and changes in the expression of SIRT3, LSP1, HRAS, SCUBE2 and AP2A2 genes in women with breast cancer in the Iranian population and findings confirmed potential biomarker and prognostic capability of these genes. Such expression profiling data can critically improve prognosis and treatment decisions in cancer patients.

Highlights

Breast cancer (BC) is one of the most common forms of cancers in Iran and is becoming the cause of most cancer-related deaths among Iranian women (Taghavi et al, 2012)
The objective of this study was to examine the gene expression profiles of SIRT3, HRAS, LSP1, SCUBE2 and AP2A2 in Iranian women with BC.A total of 136 patients including healthy controls were categorized into three groups based on the relapse of the disease
We investigated the expression of LSP1, HRAS, SIRT3, AP2A2 and SCUBE2 in Iranian BC patients and healthy control subjects

Summary

Introduction

Breast cancer (BC) is one of the most common forms of cancers in Iran and is becoming the cause of most cancer-related deaths among Iranian women (Taghavi et al, 2012). There has been a rise in the use of multigene expression assays for predicting BC prognosis This is because multigene expression profiling provides highly specific information and complements standard clinical practices and histopathological techniques, supporting clinicians in making critical treatment decisions (Patani et al, 2013). In addition to various hormonal changes and lifestyle factors, genetic factors strongly contribute to the risk of developing cancers (Cancer, 2001; Cancer, 2002; Easton, 2002). Compared to those with no family history, women with a first-degree relative affected by BC are at an estimated two-fold increased risk of BC (Cancer, 2001)

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