Abstract
Previously, in the strain Streptomyces rimosus ATCC10970 (producer of oxytetracycline), the aminoglycoside phosphotransferase AphVIII, determining kanamycin, neomycin, and paromomycin resistance, was identified and characterized. Recently, the authors obtained the 3D structure of AphVIII. The 14 aph genes, including gene aphVIII, were annotated when the genome of S. rimosus ATCC10970 was sequenced. In the present study, a new aph(3'')-Id (aphSR3) gene encoding streptomycin phosphotansferase was first identified in the strain of S. rimosus ATCC10970 using bioinformatic and comparative phylogenetic analysis of the aphSR1-aphSR14 genes with the previously known aph genes from clinical isolates and producer strains of aminoglycoside antibiotics belonging to seven subfamilies. When cloning, it was found that the gene aphSR3 (aph(3'')-Id) in Escherichia coli causes resistance to streptomycin at a concentration of 150 μg/mL. The obtained data can be used in practical terms to study the distribution and features of the functions of genes that determine the natural resistance to aminoglycoside antibiotics in actinobacteria of the genus Streptomyces.
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
